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Vent DNA polymerase

DNA polymerase

Identifiers

Organism

Thermococcus litorali

Symbol

pol

UniProt

P30317

Search for
Structures	Swiss-model
Domains	InterPro

Vent polymerase is a archean thermostable DNA polymerase used for the polymerase chain reaction. It was isolated from the thermophile Thermococcus litoralis.^[1]

References

↑ Vigneault F, Drouin R (2005). "Optimal conditions and specific characteristics of Vent exo- DNA polymerase in ligation-mediated polymerase chain reaction protocols". Biochem Cell Biol. 83 (2): 147–65. Bibcode:2005BCB....83..147V. doi:10.1139/o04-134. PMID 15864324.

v
t
e

Transferases: phosphorus-containing groups (EC 2.7)

2.7.1–2.7.4:
phosphotransferase/kinase
(PO₄)

2.7.1: OH acceptor	Hexo- Gluco- Fructo- Hepatic Galacto- Phosphofructo- 1 Liver Muscle Platelet 2 Riboflavin Shikimate Thymidine ADP-thymidine NAD⁺ Glycerol Pantothenate Mevalonate Pyruvate Deoxycytidine PFP Diacylglycerol Phosphoinositide 3 Class I PI 3 Class II PI 3 Sphingosine Glucose-1,6-bisphosphate synthase
2.7.2: COOH acceptor	Phosphoglycerate Aspartate kinase Glutamate 5-kinase
2.7.3: N acceptor	Creatine
2.7.4: PO₄ acceptor	Phosphomevalonate Adenylate Nucleoside-diphosphate Uridylate Guanylate Thiamine-diphosphate

2.7.6: diphosphotransferase
(P₂O₇)

Ribose-phosphate diphosphokinase
Thiamine diphosphokinase

2.7.7: nucleotidyltransferase
(PO₄-nucleoside)

Polymerase

DNA polymerase	DNA-directed DNA polymerase I/A γ θ ν T7 Taq II/B α δ ε ζ Pfu III/C IV/X β λ μ TDT V/Y η ι κ RNA-directed DNA polymerase Reverse transcriptase Telomerase
RNA polymerase	Template-directed RNA polymerase I II III IV V ssRNAP POLRMT Primase 1 2 PrimPol RNA-dependent RNA polymerase Polyadenylation PAP PNPase

Phosphorolytic
3′ to 5′ exoribonuclease

RNase PH
PNPase

Nucleotidyltransferase

UTP—glucose-1-phosphate uridylyltransferase
Galactose-1-phosphate uridylyltransferase

Guanylyltransferase

mRNA capping enzyme

Other

Recombinase (Integrase)
Transposase

2.7.8: miscellaneous

Phosphatidyltransferases	CDP-diacylglycerol—glycerol-3-phosphate 3-phosphatidyltransferase CDP-diacylglycerol—serine O-phosphatidyltransferase CDP-diacylglycerol—inositol 3-phosphatidyltransferase CDP-diacylglycerol—choline O-phosphatidyltransferase
Glycosyl-1-phosphotransferase	N-acetylglucosamine-1-phosphate transferase

2.7.10–2.7.13: protein kinase
(PO₄; protein acceptor)

2.7.10: protein-tyrosine	see tyrosine kinases
2.7.11: protein-serine/threonine	see serine/threonine-specific protein kinases
2.7.12: protein-dual-specificity	see serine/threonine-specific protein kinases
2.7.13: protein-histidine	Protein-histidine pros-kinase Protein-histidine tele-kinase Histidine kinase

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Polymerase chain reaction techniques

Procedure

Initialization
Cycle
Final elongation
Final hold

Polymerase

DNA-dependent	Klenow T4 and T7 Taq Tth Pfu/Pwo Tfu Tli/Vent
RNA-dependent	Viral AMV MMLV Tth RTX

Optimization
and variants

Quantitative polymerase chain reaction (qPCR)
Reverse transcription polymerase chain reaction (RT-PCR)
Inverse polymerase chain reaction (inverse PCR)
Nested polymerase chain reaction (nested PCR)
Touchdown polymerase chain reaction
Hot start PCR
Overlap extension polymerase chain reaction (OE-PCR)
Multiplex polymerase chain reaction (multiplex PCR)
Multiplex ligation-dependent probe amplification (MLPA)
Co-amplification at lower denaturation temperature PCR (COLD-PCR)
Digital polymerase chain reaction (dePCR)
Random amplification of polymorphic DNA (RAPD)

History
and people

Kary Mullis
Kjell Kleppe
Alice Chien

Portal:

Biology

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