As a true freshman with the Panthers in 2021, Bartholomew caught 28 passes for 326 yards and four touchdowns.[4] He was named honorable mention All-Atlantic Coast Conference (ACC) and was chosen second-team Freshman All-American for his performance.[1] He played in 13 games, 10 as a starter, in 2022, posting 21 catches for 283 yards and two touchdowns, and he then caught 18 passes for 326 yards and one touchdown in the 2023 season, during which he started all 10 games in which he appeared.[1] As a senior in 2024, he served as a team captain and caught 38 passes for 322 yards and four touchdowns.[5][6] He concluded his collegiate career having started 41 games and totaled 104 receptions for 1,248 yards and 11 touchdowns.[6] He accepted an invite to the 2025 Senior Bowl.[7]
Bartholomew was selected by the Minnesota Vikings in the sixth round (202nd overall) of the 2025 NFL draft.[10] He did not make a regular-season appearances for Minnesota as the result of a back injury.[11]