In a supersymmetric theory, each nonzero energy eigenvalue contains an equal number of bosonic and fermionic states. Because of this, the Witten index is independent of the temperature and gives the number of zero energy bosonic vacuum states minus the number of zero energy fermionic vacuum states. In particular, if supersymmetry is spontaneously broken then there are no zero energy ground states and so the Witten index is equal to zero.
It is an example of a quasi-topological quantity, which is a quantity that depends only on F-terms and not on D-terms in the Lagrangian. A more refined invariant in 2-dimensional theories, constructed using only the right-moving part of the fermion number operator together with a 2-parameter family of variations, is the elliptic genus.