The race started and finished in Siena, Italy. Taking place over 213 kilometres (132mi), the course included 81.7 kilometres (50.8mi) of 'strade bianche' gravel roads spread over thirteen sectors, with one new sector starting in Serravalle.[2][3] Although taking place over a similar distance to previous editions of the race, the Serravalle sector added an additional 10 kilometres (6.2mi) of 'strade bianche' gravel roads compared to the 2024 edition of the race.[2] The race finished on the Piazza del Campo, after a steep climb up Via Santa Caterina with a maximum gradient of 16%.[2]
