Scanning electron cryomicroscopy (CryoSEM) is a form of electron microscopy where a hydrated but cryogenically fixed sample is imaged on a scanning electron microscope's cold stage in a cryogenic chamber. The cooling is usually achieved with liquid nitrogen.[1] CryoSEM of biological samples with a high moisture content can be done faster with fewer sample preparation steps than conventional SEM. In addition, the dehydration processes needed to prepare a biological sample for a conventional SEM chamber create numerous distortions in the tissue leading to structural artifacts during imaging.[2][3][4][5]
↑Lesemann, Dietrich-Eckhardt; Mendgen, Kurt, eds. (1991). Electron Microscopy of Plant Pathogens. Berlin, Heidelberg: Springer Berlin Heidelberg. ISBN978-3-642-75818-8.
↑Schatten, Heide (2013). Scanning electron microscopy for the life sciences (Online-Ausg.ed.). Cambridge: Cambridge University Press. ISBN9780521195997.
↑Schatten, Heide; Pawley, James, eds. (2007). Biological low voltage field emission scanning electron microscopy. New York: Springer. ISBN9780387729725.
